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A brief review of genome editing technology for generating animal models
Haoyi WANG, Sen WU, Mario R. CAPECCHI, Rudolf JAENISCH
《农业科学与工程前沿(英文)》 2020年 第7卷 第2期 页码 123-128 doi: 10.15302/J-FASE-2019309
The recent development of genome editing technologies has given researchers unprecedented power to alter DNA sequences at chosen genomic loci, thereby generating various genetically edited animal models. This mini-review briefly summarizes the development of major genome editing tools, focusing on the application of these tools to generate animal models in multiple species.
Methodologies for the establishment of an orthotopic transplantation model of ovarian cancer in mice
null
《医学前沿(英文)》 2014年 第8卷 第1期 页码 101-105 doi: 10.1007/s11684-014-0315-5
This study used different methods to establish an animal model of orthotopic transplantation for ovarian cancer to provide an accurate simulation of the mechanism by which tumor occurs and develops in the human body. We implanted 4T1 breast cancer cells stably-transfected with luciferase into BALB/c mice by using three types of orthotopic transplantation methodologies: (1) cultured cells were directly injected into the mouse ovary; (2) cell suspension was initially implanted under the skin of the mouse neck; after tumor mass formed, the tumor was removed and ground into cell suspension, which was then injected into the mouse ovary; and (3) a subcutaneous tumor mass was first generated, removed, and cut into small pieces, which were directly implanted into the mouse ovary. After these models were established, in vivo luminescence imaging was performed. Results and data were compared among groups. Orthotopic transplantation model established with subcutaneous tumor piece implantation showed a better simulation of tumor development and invasion in mice. This model also displayed negligible response to artificial factors. This study successfully established an orthotopic transplantation model of ovarian cancer with high rates of tumor formation and metastasis by using subcutaneous tumor pieces. This study also provided a methodological basis for future establishment of an animal model of ovarian cancer in humans.
Highlights for special issue on “Large Animal Stem Cells”
Jianyong HAN
《农业科学与工程前沿(英文)》 2019年 第6卷 第1期 页码 1-2 doi: 10.15302/J-FASE-2019251
Cryopreservation of farm animal gametes and embryos: recent updates and progress
Zhengyuan HUANG, Lei GAO, Yunpeng HOU, Shien ZHU, Xiangwei FU
《农业科学与工程前沿(英文)》 2019年 第6卷 第1期 页码 42-53 doi: 10.15302/J-FASE-2018231
Cryopreservation has undergone tremendous advances and is widely used in animal production based on decades of study of cellular permeability, freezability and empirical generalization. Several improvement are particularly important: the cryopreservation protocol has been continuously refined over the years to achieve greater reproductive performance; cryoprotective agents are more effective and less toxic than previously; there has been significant innovation in advanced cryopreservation systems and carriers. Despite this, there are still problems that urgently require practical solutions, such as remedies for cryodamage and encouraging the use of frozen–thawed porcine sperm in pig production.
关键词: vitrification gametes embryo animal production cryoprotective agent freezability
Qi Lin, Xin Xu, Lihua, Wang, Qian Chen, Jing Fang, Xiaodong Shen, Liping Lou, Guangming Tian
《环境科学与工程前沿(英文)》 2017年 第11卷 第3期 doi: 10.1007/s11783-017-0924-8
关键词: Animal manure Biochar Metals Molecular species Pyrolysis
Highlights of special issue on Animal Science
Jun REN
《农业科学与工程前沿(英文)》 2017年 第4卷 第3期 页码 257-259 doi: 10.15302/J-FASE-2017165
Antimicrobial use in food animal production: situation analysis and contributing factors
Ziping WU
《农业科学与工程前沿(英文)》 2018年 第5卷 第3期 页码 301-311 doi: 10.15302/J-FASE-2018207
By measuring the quantity and ways in which antimicrobials are used, and reviewing different technical and socioeconomic factors influencing antimicrobial use at farm level, this study discusses the main knowledge gaps in antimicrobial use in food animal production and provides recommendations for future research and policy development. The review reveals that antimicrobial use in food animals exhibit strong regional and species differences, and there are still large information gaps concerning the current state of antimicrobial use. Factors associated with animal health (including antimicrobial resistance), animal health improvement, economic costs and benefits relevant to animal diseases, and potential technological alternatives or alternative systems all have an impact on antimicrobial use on the farm. There is a clear need to resolve the data gap by monitoring antimicrobial use and developing an analytical framework to better understand farmer behaviors under different technical, economic and environmental circumstances.
关键词: antimicrobial use drivers for antimicrobial use food animal production
Electrolyzed water and its application in animal houses
Weichao ZHENG,Li NI,Baoming LI
《农业科学与工程前沿(英文)》 2016年 第3卷 第3期 页码 195-205 doi: 10.15302/J-FASE-2016109
关键词: disinfection poultry and livestock slightly acidic electrolyzed water neutral electrolyzed water
Animal stem cells: the engineering development front of 2018
Wenye YUAN, Jianxiang XU, Chenying LI, Lijuan SHI, Aidong YANG, Yan WANG, Lu XING, Shaozhong KANG
《农业科学与工程前沿(英文)》 2019年 第6卷 第1期 页码 93-95 doi: 10.15302/J-FASE-2018250
陈松林,徐文腾,陈张帆
《中国工程科学》 2019年 第21卷 第6期 页码 39-47 doi: 10.15302/J-SSCAE-2019.06.007
本文主要从基因组和转录组层面分析了极地动物基因资源的研究现状,梳理了本领域研究中存在的问题,并提出了未来发展战略。极地动物基因组测序起步较晚,迄今只完成了 13种极地动物的全基因组测序。在转录组研究方面,人们对极地的 31个物种进行了转录组测序,并在以下四个方向重点开展了研究:环境适应性研究;污染物应激反应的分子机制研究;不同发育阶段或不同组织中的转录组分析;功能基因挖掘。本领域研究由于起步晚,研究广度和深度都有待加强,但极地动物的基因资源研究具有战略意义。建议国家设立“极地动物基因资源发掘与应用”重点研发计划专项,围绕极地渔业动物特殊性状遗传解析、特有基因功能分析和基因工程产品研发等开展研究。
关键词: 极地动物 基因组,转录组,基因资源
Comparison of analytical procedures for measuring phosphorus content of animal manures in China
null
《农业科学与工程前沿(英文)》 2019年 第6卷 第4期 页码 431-440 doi: 10.15302/J-FASE-2019279
The concentration and components of manure phosphorus (P) are key factors determining potential P bioavailability and runoff. The distribution of P forms in swine, poultry and cattle manures collected from intensive and extensive production systems in several areas of China was investigated with sequential fractionation and a simplified two-step (NaHCO -NaOH/EDTA) procedures. The mean total P concentration, determined by the sequential fractionation procedure of intensive swine, poultry and cattle manure, expressed as g·kg , was 14.9, 13.4 and 5.8 g·kg , respectively, and 4.4 g·kg in extensive cattle manure. In intensive swine, poultry and cattle manure about 73%, 74% and 79% of total P, respectively, was bioavailable (i.e., P extracted by H O and NaHCO ) and 78% in extensive cattle manure. The results indicated the relative environmental risk, from high to low, of swine, poultry and cattle manure. There is considerable regional variation in animal manure P across China, which needs to be considered when developing manure management strategies.
关键词: diet phosphorus manure phosphorus sequential P fractionation
Mengshi LIN,
《化学科学与工程前沿(英文)》 2009年 第3卷 第4期 页码 427-435 doi: 10.1007/s11705-009-0244-z
关键词: milk-derived Melamine labor-intensive cyanurate time-consuming
Xingfan ZHOU,Zengling YANG,Longjian CHEN,Lujia HAN
《农业科学与工程前沿(英文)》 2016年 第3卷 第2期 页码 171-179 doi: 10.15302/J-FASE-2016095
关键词: self-organizing feature maps visualization processed animal proteins (PAPs) amino acid
标题 作者 时间 类型 操作
A brief review of genome editing technology for generating animal models
Haoyi WANG, Sen WU, Mario R. CAPECCHI, Rudolf JAENISCH
期刊论文
Methodologies for the establishment of an orthotopic transplantation model of ovarian cancer in mice
null
期刊论文
Cryopreservation of farm animal gametes and embryos: recent updates and progress
Zhengyuan HUANG, Lei GAO, Yunpeng HOU, Shien ZHU, Xiangwei FU
期刊论文
The speciation, leachability and bioaccessibility of Cu and Zn in animal manure-derived biochar: effect
Qi Lin, Xin Xu, Lihua, Wang, Qian Chen, Jing Fang, Xiaodong Shen, Liping Lou, Guangming Tian
期刊论文
Antimicrobial use in food animal production: situation analysis and contributing factors
Ziping WU
期刊论文
Animal stem cells: the engineering development front of 2018
Wenye YUAN, Jianxiang XU, Chenying LI, Lijuan SHI, Aidong YANG, Yan WANG, Lu XING, Shaozhong KANG
期刊论文
Comparison of analytical procedures for measuring phosphorus content of animal manures in China
null
期刊论文
A review of traditional and novel detection techniques for melamine and its analogues in foods and animal
Mengshi LIN,
期刊论文
Visualization of amino acid composition differences between processed protein from different animal species
Xingfan ZHOU,Zengling YANG,Longjian CHEN,Lujia HAN
期刊论文